Bi-Chang Chen 陳壁彰
Assistant Research Fellow

Ways to contact me:
Phone:02-2787-3133 or 2787-3152
Fax: 02-2787-3122
Address: Research Center for Applied Sciences, Academia Sinica
128 Sec. 2, Academia Rd., Nankang, Taipei 11529, Taiwan
Job Opening : At all levels



Research Fields:

  • Light Sheet Microscopy
  • Developing fast, low phototoxicity, multi-color, and 3D detection for fluorescent living specimens with subcellular resolution imaging tools
  • Applications ranging from single molecule tracking to high spatiotemporal imaging of subcellular dynamics within whole developing embryos.

Recent Publications:

  1. Yang,W.-T.; Li,Y.-C.; Lin,C.-M.; Tang,W.-C.; Chen, B.-C.; Emiko Suzuki,; Hsu,J.-C., Adherens junction-associated pores mediate the intercellular transport of endosomes and cytoplasmic proteins,Biochemical and Biophysical Research Communications,505(2018)
    [ DOI:10.1016/j.bbrc.2018.09.129 ]
  2. Chatterjee,; Krishnendu,; Feby Wijaya Pratiwi,; Frances Camille M.; Chen,P.-L.; and Chen, B.-C., “Recent Progress in Light Sheet Microscopy for Biological Applications.” Applied Spectroscopy,72,1137–1169(2018)
    [ DOI:10.1177/0003702818778851 ]
  3. Chiang,Y.-Y.; Tsou,C.-F.; Chen, B.-C.; Chou,R.-H.; "Glass-based microchip with finger electrodes for the formation, collection, and biointeractions of liposomes," Journal of Micro/Nanolithography, MEMS, and MOEMS,17(2), 025001.(2018)
    [ DOI:10.1117/1.JMM.17.2.025001 ]
  4. Cai, E., K. Marchuk, P. Beemiller, C. Beppler, M. G. Rubashkin, V. M. Weaver, A. Gérard, Liu,T.-L.; Chen, B.-C.; E. Betzig, F. Bartumeus and M. F. Krummel. "Visualizing dynamic microvillar search and stabilization during ligand detection by T cells." Science 356(6338).(2017)
    [ DOI:10.1126/science.aal3118 ]
  5. Fritz-Laylin, L. K.; Riel-Mehan, M.; Chen, B.-C.; Lord, S. J.; Goddard T. D.; Ferrin, T. E.; Nicholson-Dykstra, S. M.; Higgs, H.; Johnson G.T.; Betzig, E,; Mullins, R. D. Actin-based protrusions of migrating neutrophils are intrinsically lamellar and facilitate direction changes, eLife, 6:e26990 (2017)
    [ DOI:10.7554/eLife.26990 ]
  6. Veltman, D. M.; Williams, T. D.; Bloomfield, G; Chen, B.-C.; Betzig, E.; Insall R.H. and Kay, R. R.,A plasma membrane template for macropinocytic cups, eLife, 5:e20085 (2016)
    [ DOI:10.7554/eLife.20085 ]
  7. Jung, G; Alexander, C. J.; Wu, X.; Piszczek, G; Chen, B.-C.; Betzig, E. and Hammer, J. A.,V-1 Regulates Capping Protein Activity in Vivo PNAS, 113, E6610–E6619 (2016)
    [ DOI:10.1073/pnas.1605350113 ]
  8. Aguet, F.; Upadhyayula, S.; Gaudin, R.; Chou, Y-Y, Cocucci,E; He, H; ,Chen,B.-C.; Mosaliganti, K; Pasham, M; Skillern, W; Legant, W.; Liu,T.-L.; Findlay, G; Marino, E; Danuser,G; Megason, S; Betzig, E and Kirchhausen, T, Membrane dynamics of dividing cells imaged by lattice light-sheet microscopy Molecular Biology of the Cell, 27, 3418-3435 (2016)
    [ DOI:10.1091/mbc.E16-03-0164 ]
  9. Chin, L.; Lee, C.-H.; Chen, B.-C.. Imaging Live at High Spatiotemporal Resolution for Lab-on-a-Chip Applications, Lab on a Chip , 16, 2014-2024 (2016)

    [ DOI:10.1039/C5LC01556A ]
  10. Yamashita, N.; Morita, M.; Legant, W. R.; Chen, B.-C.; Betzig, E.; Yokoto, H.; Mimori-Kiyosue, Y., Three-dimensional tracking of plus-tips by lattice light-sheet microscopy permits the quantification of microtubule growth trajectories within the mitotic apparatus, J. Biomed. Opt., 20, 101206.(2015)
    [ DOI:10.1117/1.JBO.20.10.101206 ]
  11. Xie, J.; Wooten, M.; Tran, V.; Chen, B.-C.; Pozmanter, C.; Simbolon, C.; Betzig, E.; Chen, X., Histone H3 Threonine Phosphorylation Regulates Asymmetric Histone Inheritance in the Drosophila Male Germline. Cell, 163, 920-933, (2015)
    [ DOI:10.1016/j.cell.2015.10.002 ]
  12. Li, D.; Shao, L.;Chen, B.-C.; Zhang, X.; Zhang, M.; Moses, B.; Milkie, D. E.; Beach, J. R.; Hammer III J. A.; Pasham M.; Kirchhausen, T.; Baird M. A.; Davidson, M. W.; Xu, P.; Betzig, E., Extended resolution structured illumination imaging of endocytic and cytoskeletal dynamics. Science, 349, 6251 (2015)
    [ DOI:10.1126/science.aab3500 ]
  13. Thievessen, I.; Fakhri, N.; Steinwachs, J.; Kraus, V.; Mclsaac R. S.; Gao, L.;Chen, B.-C.; Baird, M. A.; Davidson, M. W.; Betzig, E.; Oldenbourg, R.; Waterman, C.M.; Fabry, B., Vinculin is required for cell polarization, migration, and ECM-remodeling in 3D collagen. FASEB J., pii:fj.14-268235 (2015)
    [ DOI:10.1096/fj.14-268235fj.14-268235 ]
  14. Li, Y.-C.; Yang, W.-T.; Cheng, L.-C.; Lin, Y.-H.; Ho, Y.-H.; Lin, P.-Y.;Chen, B.-C.; Rickoll, W. L.; Hsu, J.-C., Novel transport function of adherens junction revealed by live imaging in Drosophila. Biochem Biophys Res Commun., 463(4):686-92 (2015)
    [ DOI:10.1016/j.bbrc.2015.05.125. ]
  15. Kural, C.; Akatay, AA.; Gaudin, R.; Chen, B.-C.; Legant, WR.; Betzig, E.; Kirchhausen, T.,Asymmetric formation of coated pits on dorsal and ventral surfaces at the leading edges of motile cells and on protrusions of immobile cells.Molecular Biology of the Cell, 26,2044-2053(2015)
    [ DOI:10.1091/mbc.E15-01-0055 ]
  16. Ritter, A. T.; Asano, Y.; Stinchcombe J. C.; Dieckmann, N. M. G.; Chen, B.-C. ; Schuyler G. C.; Legant, W.; Gao, L.; Davidson, M. W.; Betzig, E.; Lippincott-Schwartz, J; Griffiths G. M., Actin depletion initiates events leading to granule secretion at the immunological synapse. Immunity, 42, 864-876 (2015)
    [ DOI:10.1016/j.immuni.2015.04.013 ]
  17. Wang, J. T.; Smith, J.; Chen, B.-C.; Schmidt, H.; Rasoloson, D.; Paix, A.; Lambrus, B. G; Calidas, D.; Betzig, E.; Seydoux, G., Regulation of RNA granule dynamics by phosphorylation of serine-rich, intrinsically-disordered proteins in C. elegans. eLife, DOI : 10.7554/eLife.04591 (2014).
    [ DOI:10.7554/eLife.04591 ]
  18. Liu, Z.; Legant, W.; Chen, B.-C.; Li, L.; GrimmMilkie, J. B., Lavis, L. D.; Betzig, E.; Tjian, R, 3D Imaging of Sox2 Enhancer Clusters in Embryonic Stem Cells. eLife, DOI : 10.7554/eLife.04236 (2014).
    [ DOI:10.7554/eLife.04236 ]
  19. Chen, B.-C.; Legant, W.; Wang, K; Shao, L.; Milkie, D. E.; Davidson, M. W.; Janetopoulos, C.; Wu, X. S.; Hammer III, J. A.; Liu, Z.; English, B. P.; Mimori-Kiyosue, Y.; Ritter, A.; Lippincott-Schwartz, J; Fritz-Laylin, L.; Mullins, R. D.; Mitchell, D.; Bembenek, J. M.; Reyman, A-C.; Bohme, R.; Grill, S. W.; Wang, J.; Seydoux, G.; Tulu, U. S.; Kiehart, D. P.; Betzig, E., Lattice Light Sheet Microscopy: Imaging Molecules to Embryos at High Spatiotemporal Resolution . Science, 346, 1257998/1-1257998/12 (2014).
    [ DOI:10.1126/science.1257998 ]
  20. Chen, J.; Li, L.; Zhang, Z.; Chen, B.-C.; Revyakin, A.; Hajj, B.; Legant, W.; Dahan, M.; Lionnet, T.; Betzig, E.; Tjian, R.; Liu, Z., Single-molecule Dynamics of Enhanceosome Assembly in Embryonic Stem Cells, Cell, 156, 1274-1285 (2014).
    [ DOI:10.1016/j.cell.2014.01.062 ]
  21. Gao, L. ; Shao, L.; Chen, B.-C.; Betzig, E., 3D Live Fluorescence Imaging of Cellular Dynamics Using Bessel Beam Plane Illumination Microsocpy. Nature Protocols , 9, 1083-1101 (2014). Selected for the cover article
    [ DOI:10.1038/nprot.2014.087 ]
  22. Habib, S. J.; Chen, B.-C.; Tsai, F.-C.; Anastassiadis, K.; Meyer, T.; Betzig, E.;Nusse R., A Localized Wnt Signal Orients Asymmetric Stem Cell Divison in Vitro. Science, 22, 1445-1448 (2013).
    [ DOI:10.1126/science.1231077 ]
  23. Sung, J.; Chen, B.-C.; Lim, S.-H., Fast Three-Dimensional Chemical Iimaging by Interferometric Multiplex Coherent Anti-Stokes Raman Scattering Microscopy. Journal of Raman Spectroscopy, 42, 130-136 (2011).
    [ DOI:10.1002/jrs.2647 ]
  24. Chen, B.-C.; Sung, J.; Wu, X.; Lim, S.-H., Chemical Imaging and Microspectroscopy with Spectral Focusing Coherent Anti-Stokes Raman Scattering (CARS). Journal of Biomedical Optics, 16, 021112 (2011).
    [ DOI:10.1117/1.3533315 ]
  25. Chen, B.-C.; Sung, J.; Lim, S.-H., Chemical Imaging with Frequency Modulation Coherent Anti-Stokes Raman Scattering microscopy at the vibrational Fingerprint Region. Journal of Physical Chemistry B, 114, 16871-16880 (2010).
    [ DOI:10.1021/jp104553s ]
  26. Chen, B.-C.; Lim, S.-H., Three-Dimensional Imaging of Direct field Orientations in Liquid Crystals by Polarized Four-Wave Mixing Microscopy. Applied Physics Letters, 94, 171911/1-171911/3 (2009).
    [ DOI:10.1063/1.3127535 ]
  27. Sung, J.; Chen, B.-C.; Lim, S.-H., Single-beam Homodyne SPIDER for Multiphoton Microscopy. Optics Letters, 33, 1404-1406 (2008). Selected for Virtual Journal of Ultrafast Science
    [ DOI:10.1364/OL.33.001404 ]
  28. Chen, B.-C.; Lim, S.-H., Optimal Laser Pulse Shaping for Interferometric Multiplex Coherent Anti-Stokes Raman Scattering Microscopy. Journal of Physical Chemistry B, 112, 3653-3661 (2008). Selected for the cover article
    [ DOI:10.1021/jp710743k ]
  29. Chen, B.-C.; Lim, S.-H., Characterization of a Broadband for Phased controlled Multiphoton Microscopy by Single Beam SPIDER. Optics Letter, 32, 2411-2413 (2007). Selected for Virtual Journal of Ultrafast Science.
    [ DOI:10.1364/OL.32.002411 ]
  30. Yeh, Y.-Q.; Chen, B.-C.; Lin, H.-P.; Tang, C.-Y., Synthesis of Hollow silica Spheres with Mesostructured Shell Using Cational-Anionic-Neutral Block Copolymer Ternary Surfactants. Langmuir, 22, 6-9 (2006).
    [ DOI:10.1021/la052129y ]
  31. Chen, B.-C.; Chao, M.-C.; Lin, H.-P; Mou, C.-Y., Faceted single Crystals of Mesoporous Silica SBA-16 from a Ternary Surfactant System : surface Roughening Model, Microporous and Mesoporous Material, 81, 241-249 (2005).
    [ DOI:10.1016/j.micromeso.2005.02.010 ]
  32. Chen, B.-C.; Lin, H.-P.; Chao, M.-C.; Mou, C.-Y.; Tang, C.-Y., Mesoporous silica Platelets with Perpendicular Nanochannel via a Ternary-Surfactant Template. Advanced Materials, 16, 1657-1661 (2004).
    [ DOI:10.1002/adma.200306327 ]