Chau-Hwang Lee 李超煌
Research Fellow

Ways to contact me:
Fax: 886-2-27873122
Address: Research Center for Applied Sciences, Academia Sinica
128 Sec. 2, Academia Rd., Nankang, Taipei 11529, Taiwan



Research Fields:

  • Cell-cell and cell-microenvironment interactions.
  • Super resolution far-field optical microscopy.
  • Differential sectioning microscopy.
  • Mechanical and electrical characteristics of bio-membranes.
  • Laser-assisted micro/nano fabrication.

Recent Publications:

  1. Y.-C. Kao, J.-R. Jheng, H.-J. Pan, W.-Y. Liao, C.-H. Lee, and P.-L. Kuo, "Elevated hydrostatic pressure enhances the motility and enlarges the size of the lung cancer cells through aquaporin upregulation mediated by caveolin-1 and ERK1/2 signaling," Oncogene 36, 863 (2017).
  2. L. K. Chin, C.-H. Lee, and B.-C. Chen, "Imaging live cells at high spatiotemporal resolution for lab-on-a-chip applications," Lab on a Chip 16, 2014 (2016).
  3. P. Y. Liu, L. K. Chin, W. Ser, H. F. Chen, C.-M. Hsieh, C.-H. Lee, K.-B. Sung, T. C. Ayi, P. H. Yap, B. Liedberg, K. Wang, T. Bourouinaj, and Y. Leprince-Wang, "Cell refractive index for cell biology and disease diagnosis: past, present and future," Lab on a Chip 16, 634 (2016)
  4. C.-W. Lee, L.-L. Jang, H.-J. Pan, Y.-R. Chen, C.-C. Chen, and C.-H. Lee, "Membrane roughness as a sensitive parameter reflecting the status of neuronal cells in response to chemical and nanoparticle treatments," Journal of Nanobiotechnology 14, 9 (2016).
  5. B. Patra, C.-C. Peng, W.-H. Liao, C.-H. Lee, and Y.-C. Tung, "Drug testing and flow cytometry analysis on a large number of uniform sized tumor spheroids using a microfluidic device," Scientific Reports 6, 21061 (2016).
  6. Y.-H. Su, P.-C. Chiang, L.-J. Cheng, C.-H. Lee, N. S. Swami, and C.-F. Chou, "High aspect ratio nanoimprinted grooves of poly(lactic-co-glycolic acid) control the length and direction of retraction fibers during fibroblast cell division," Biointerphases 10, 041008 (2015).
  7. C.-C. Lan, E. Y. Lu, H.-J. Pan, and C.-H. Lee, "Directional migration of cancer cells induced by a blue light intensity gradient," Biomedical Optics Express 6, 2624 (2015).
  8. Y.-F. Hsiao, H.-J. Pan, Y.-C. Tung, C.-C. Chen, and C.-H. Lee, "Effects of hydraulic pressure on cardiomyoblasts in a microfluidic device," Biomicrofluidics 9, 024111 (2015).
  9. W.-Y. Liao, C.-C. Ho, H.-H. Hou, T.-H. Hsu, M.-F. Tsai, K.-Y. Chen, H.-Y. Chen, Y.-C. Lee, C.-J. Yu, C.-H. Lee, and P.-C. Yang, "Heparin cofactor II enhances cell motility and promotes metastasis in non-small-cell lung cancer," Journal of Pathology 235, 50 (2015).
  10. B. Patra, Y.-S. Peng, C.-C. Peng, W.-H. Liao, Y.-A. Chen, K.-H. Lin, Y.-C. Tung, and C.-H. Lee, "Migration and vascular lumen formation of endothelial cells in cancer cell spheroids of various sizes," Biomicrofluidics 8, 052109 (2014).
  11. Y.-C. Kao, M.-H. Hsieh, C.-C. Liu, H.-J. Pan, W.-Y. Liao, J.-Y. Cheng, P.-L. Kuo, and C.-H. Lee, "Modulating chemotaxis of lung cancer cells by using electric fields in a microfluidic device," Biomicrofluidics 8, 024107 (2014).
  12. Y.-R. Liou, W. Torng, Y.-C. Kao, K.-B. Sung, C.-H. Lee, and P.-L. Kuo, "Substrate stiffness regulates filopodial activities in lung cancer cells,” PLoS ONE 9, e89767 (2014).
  13. L.-W. Chu, Y.-L. Huang, J.-H. Lee, L.-Y. Huang, W.-J. Chen, Y.-H. Lin, J.-Y. Chen, R. Xiang, C.-H. Lee, and Y.-H. Ping, "Single-virus tracking approach to reveal the interaction of Dengue virus with autophagy during the early stage of infection," Journal of Biomedical Optics 19, 011018 (2014).
  14. H.-J. Pan, R.-L. Wang, J.-L. Xiao, Y.-J. Chang, J.-Y. Cheng, Y.-R. Chen, and C.-H. Lee, "Using optical profilometry to characterize cell membrane roughness influenced by amyloid-beta 42 aggregates and electric fields," Journal of Biomedical Optics 19, 011009 (2014).
  15. B. Patra, Y.-H. Chen, C.-C. Peng, S.-C. Lin, C.-H. Lee, and Y.-C. Tung, "A microfluidic device for uniform-sized cell spheroids formation, culture, harvesting and flow cytometry analysis," Biomicrofluidics 7, 054114 (2013).
  16. H.-F. Tsai, C.-W. Huang, H.-F. Chang, J. J. W. Chen, C.-H. Lee, and J.-Y. Cheng, "Evaluation of EGFR and RTK signaling in the electrotaxis of lung adenocarcinoma cells under direct-current electric field stimulation," PLoS ONE 8, e73418 (2013).
  17. J.-L. Xiao, D.-H. Lu, and C.-H. Lee, "Guiding the migration of adherent cells by using optical micropatterns," Applied Physics Letters 102, 123703 (2013).
  18. J.-W. Huang, H.-J. Pan, W.-Y. Yao, Y.-W. Tsao, W.-Y. Liao, C.-W. Wu, Y.-C. Tung, and C.-H. Lee, "Interaction between lung cancer cell and myofibroblast influenced by cyclic tensile strain," Lab on a Chip 13, 1114 (2013).
  19. H.-Y. Hsieh, T.-W. Huang, J.-L. Xiao, C.-S. Yang, C.-C. Chang, C.-C. Chu, L.-W. Lo, S.-H. Wang, P.-C. Wang, C.-C. Chieng, C.-H. Lee, and F.-G. Tseng, "Fabrication and modification of dual-faced nano-mushrooms for tri-functional cell theranostics: SERS/fluorescence signaling, protein targeting, and drug delivering," Journal of Materials Chemistry 22, 20918 (2012).
  20. J.-L. Xiao, H.-J. Pan, and C.-H. Lee, "Optically micropatterned culture of adherent cells," Journal of Biomedical Optics 17, 075004 (2012).
  21. T.-H. Hsu, Y.-L. Kao, W.-L. Lin, J.-L. Xiao, P.-L. Kuo, C.-W. Wu, W.-Y. Liao, and C.-H. Lee, "Migration speed of cancer cells influenced by macrophages and myofibroblasts co-cultured in a microfluidic chip," Integrative Biology 4, 177 (2012).


  1. Height measurement by correlating intensity with position of scanning object along optical axis of a structured illumination microscope. US Patent 8,705,043, ROC (Taiwan) Patent I438392.
  2. Optical Sectioning Microcopy, US Patent 8,019,136, Japan Patent 5085608, ROC (Taiwan) Patent I414818.
  3. Modulation Differential Confocal Microscopy, ROC (Taiwan) Patent I329207.
  4. Method for Characterizing Transparent Thin-films Using Differential Optical Sectioning Interference Microscopy, US Patent 7,545,510, ROC (Taiwan) Patent I328676.
  5. Differential Confocal Microscopy, ROC (Taiwan) Patent 082212, US Patent 5,804,813.